STATE-OF-THE-ART OPTICAL AND CONFOCAL MICROSCOPY

Ferreyra Institute´s Optical Microscopy Lab was set up in conjunction with CIQUIBIC and serves as a unit dedicated to developing basic and applied research programmes, mainly in the fields of Biology, Chemistry, Physics and Medical Sciences.

Description: 

The State-of-the-art Optical and Confocal Microscopy Center offers the possibility of using high-tech fluorescence optical microscopes, such as confocal, spinning disk and TIRF for the collection of images from optical sectionings, mainly in fixed or live biological samples. These devices are used only by high-qualified professionals in charge of the area, and by those researchers with proven experience in handling such equipment.

Methodology: 

Images can be obtained in a broad spectrum of wavelengths from 405 to 633 nm; in the x, y, z, t variables. Techniques such as FRAP (Fluorescence Recovery After Photobleaching), FLIP (Fluorescence Loss in Photobleaching), FRET (Fluorescence Resonance Energy Transfer), FLIM (Fluorescence Lifetime Imaging), Spectral Deconvolution, TIRF (evanescent field microscopy) and DSU (Disk Scanning Unit) enable the study of live cellular events in high temporal resolution.

Confocal microscopy services are subject to a fee.

Person in charge:

Gonzalo Quasollo

gquassollo@gmail.com

Opening Hours: From Monday to Friday, 9 a.m. to 5 p.m.

 

MATERIAL STERILIZATION

Description: 

The service consists in sterilization by steam saturated at a pressure higher than normal for various materials and supplies (vivarium supplies such as cob, shavings, food, or those provided by the client like stainless steel instruments, textiles, borosilicate glass materials, paper, disposable products, aqueous solutions, among others.) The sterilization process is assessed through the use of parametric and biological controls in each of the cycles performed in autoclave.

Methodology: 

The STAN performs material sterilization through a physical procedure which coagulates protein structures in microorganisms by means of steam saturated at a pressure higher than normal. It is the preferred method for sterilizing materials that are moisture-insensitive and which tolerate process temperatures of between 121°C and 134°C.

People in charge:

Marisa Gigena

mgigena@immf.uncor.edu

 Romina Maiorano

rmaiorano@immf.uncor.edu

BIOLOGICAL SAMPLES AND INORGANIC SOLUTIONS LYOPHILIZATION

Description:  

The process consists in fractioning and freezing the sample - that can be proteins, biological value peptides or buffers, catalysts, among others - and its later lyophilization for the sample´s conservation. This provides the sample with a good protection against external influences and makes it quickly available for use.

Methodology: 

Lyophilization consists in eliminating the water from the sample through its freezing and later ice sublimation under vacuum conditions. When supplying heat, the ice sublimates and the liquid phase is avoided. It is a set of processes - not just the drying -, in which the resulting material will be dry, and all features and texture of the original product will be preserved. This is what distinguishes and makes the lyophilization process stand out from other processes used in dehydrations.

Person in charge:

Daniel Raimunda

draimunda@immf.uncor.edu

 

 

 

DETERMINING RADIOACTIVITY IN LIQUID SAMPLES

Description:  

Radioactivity detection is achieved through the use of a liquid scintillation counter that measures beta particles sent out by a compound that possesses radiation. In all cases, its manipulation is performed according to the security regulations and X-ray protections currently in force. This procedure is widely used in the Industry, Agriculture, Biomedical Sciences, Molecular Biology, Environmental Sciences and in research projects.

Methodology:  

The method consists in placing unknown solutions in glass or plastic vials containing scintillation liquid which detects and quantifies radioactivity. This detection method is useful for quantifying and identifying beta pure emitter radioisotopes. Scintillation liquids are a mixture of solvents and organic compounds with fluorescent and surfactant properties that enable the acquisition of homogeneous solutions between aqueous samples and organic scintillating solutions.

Person in charge:

Daniel Raimunda

draimunda@immf.uncor.edu

 

 

DETERMINING OSMOLARITY TO INORGANIC AND BIOLOGICAL SOLUTIONS

Description: 

This service involves measuring osmolarity to solutions, in other words, the solute effective concentration that generate osmotic pressure. The determination is conducted through the hygrometric method, using a steam pressure Osmometer. Osmolarity is the measurement used by biologists, pharmacists, physicians and veterinarians to express the substance total concentration in solutions used in Medical Sciences.

Methodology: 

The osmometer uses the hygrometric method for determining steam pressure. The sensitive thermocouple and sophisticated electronics provide the medium for measuring a sample´s depression of dew point temperature. The steam pressure and freezing point are among a solution´s colligative properties. In contrast to pure solvents, these properties are altered in proportion to the number of solute particles dissolved in each kilogram of solvent.

Responsable:

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