Brief summary of research lines and academic activities

Dr. Bagatolli has worked over 25 years on biophysical aspects of biological membranes. Currently his research topics are related to the physicochemical aspects of cellular systems, particularly aspects related to non-equilibrium thermodynamics, molecular crowding and spatial confinement that occurs in the intracellular environment during cell function using mainly state-of-the-art fluorescence related techniques (e.g. spectroscopy, confocal and multiphoton excitation microscopy). Dr. Bagatolli has been published numerous scientific articles, including scientific papers, review articles and book chapters, including 3 books, mostly on structural and dynamic aspects related to lipid-lipid and lipid-protein interactions of membranes of different composition (synthetic lipid mixtures, natural lipid extracts and natural occurring membranes such as those existing in the skin stratum corneum and lung surfactant).

More information (complete CV and list of publications) can be found at the following link: http://memphys.dk/people/luis/index.html

Emergent phenomena in biomimetic systems and cells.

This project aims to explore how key cellular features, such as molecular crowding (the situation in which solutes, by their nature and concentration, alter intensive properties of the aqueous environment), spatial confinement and limited availability of free water can impact the dynamical and structural characteristics of living cells during cellular function. The relevance of these parameters in the behavior of cells has been documented over the years, although paradoxically they are systematically disregarded in the canonical models that describe the cell. The specific aim of this project attempt to study in both cells and biomimetic systems, the impact of these key cellular features may regulate the coupling between mechano-chemical properties of the cell cytoplasm and oscillatory biochemical processes involving ATP metabolism. We posit that only a few fundamental physicochemical descriptors are necessary to understand integrated function in cellular systems, bringing rationality to the current descriptive schemes based on tens of thousands of molecular actors interacting without spatiotemporal information. Substantive modifications in the current premises (e.g. van’t Hoff’s ideal solution theory, mass action kinetics) that describe the function of cellular systems symbolize in my opinion the grand challenge of this ambitious endeavor.

INTERESTED IN JOINING MY LABORATORY? Send a short summary describing your interest and your CV to lbagatolli@immf.uncor.edu


LAB STUDENTS

Dr. Agustín Mangiarotti

Postdoc CONICET

Dr. Santiago Otaiaza

Postdoc CONICET

Bioq. Clara Castañares

PhD CONICET

GRANTS (Active) 

AGENCIA-FONCyT PICT-2017-2658 - Tipo A (PI, 07/2018), Argentina

PUBLICATIONS (last 5 years)

Research papers (peer reviewed)

  • A. Bagatolli. A. Mangiarotti and R.P. Stock "Cellular metabolism and colloids: realistically linking physiology and biological physical chemistry" Progress in Biophysics and Molecular Biology in press (2020) view►
  • F. Olsen, R.P. Stock and L.A. Bagatolli. 2020. Glycolytic oscillations and intracellular K+ concentration are strongly coupled in the yeast Saccharomyces cerevisiae. Arch. Biochem. Biophys. 681:108257 view►
  • A. Bagatolli, R.P. Stock and L.F. Olsen. 2019. Coupled response of membrane hydration with oscillating metabolism in live cells: an alternative way to modulate structural aspects of biological membranes? Biomolecules, 9(11), 687 view►
  • Wai Leung, J. Brewer, L.A. Bagatolli, and J.L. Thewalt. 2019. "Measuring molecular order for lipid membrane phase studies: linear relationship between Laurdan generalized polarization and deuterium NMR order parameter" Biochim. Biophys. Acta - Biomembranes, 1861(12):183053 view►
  • M Pazin, S.N.D. Santos, S.C. N. Queiroz, L.A. Bagatolli, A.E.E. Soares, I.S. Melo and A.S. Ito. 2019. Bioactivity and action mechanism of green propolis against Pythium aphanidermatum. An. Acad. Bras. Cienc. 91(2):e20180598. view►
  • Seir Thoke, L.A. Bagatolli and L. F. Olsen. 2018. "Effect of macromolecular crowding on the kinetic of glycolytic enzymes and the behaviour of glycolysis in yeast" Integrative Biology, 10(10):587-597 view►
  • Seir Thoke, L. F. Olsen, L. Duelund, R. P. Stock, T. Heimburg and L.A. Bagatolli. 2018. "Is a constant low entropy process at the root of glycolytic oscillations?" J. Biol. Physics, 44(3):419-431 view►
  • Rodi, B. Maggio, L.A. Bagatolli. 2018. "Direct visualization of the lateral structure of giant vesicles composed of pseudo- binary mixtures of sulfatide, asialo-GM1 and GM1 with POPC", Biochim. Biophys. Acta - Biomembranes, 1860(2):544–555 view►
  • Seir Thoke, S. Thorsteinsson, R.P. Stock, L.A. Bagatolli and L. Folke Olsen. 2017. "The dynamics of intracellular water constrains glycolytic oscillations in Saccharomyces cerevisiae", Scientific Reports, 7:16250 (doi:10.1038/s41598-017-16442-x) view►
  • Bavastrello, A. Caliari, I. Pesce, L.A. Bagatolli and M. Hanczyc. 2017. "Easy and fast preparation of large and giant vesicles from highly confined thin lipid films deposited at the air water interface", BioNanoScience, pp. 1-11 (doi:10.1007/s12668-017-0464-5). view►
  • Bolean, I.A. Borin, A.M.S. Simão, M. Bottini, L.A. Bagatolli, M.F. Hoylaerts, J.L. Millán, and P. Ciancaglini. 2017. “Topographic analysis by atomic force microscopy of proteoliposomes matrix vesicle mimetics harboring TNAP and AnxA5” Biochim. Biophys. Acta - Biomembranes 1859(10): 1911-1920. view►
  • Bloksgaard, T. M. Leurgans, B. Spronck, M.H.G. Heusinkveld, K. Rosenstand, I. Nissen, J. R. Brewer, L.A. Bagatolli, L.M. Rasmussen, A. Irmukhamedov, K.D. Reesink, J.G.R. De Mey. 2017. "Imaging and modeling of acute pressure-induced changes of collagen and elastin microarchitectures in pig and human resistance arteries", American Journal of Physiology-Heart and Circulatory Physiology 313(1):H164-H178. view►
  • G. Mouritsen, L. A. Bagatolli, L. Duelund, O. Garvik, J. H. Ipsen, A. Cohen Simonsen. 2017. "Effects of seaweed sterols fucosterol and desmosterol on lipid membranes" Chem. Phys. Lipids 205:1-10 view►
  • Cheniour, J. Brewer, L.A. Bagatolli, O. Marcillat and T. Granjon. 2017. "Evidence of proteolipid domain formation in an inner mitochondrial membrane mimicking model", Biochim. Biophys. Acta - General subjects, 1861:969-976 view►
  • Brewer, H.S. Thoke, R.P. Stock and L.A. Bagatolli. 2017. "Enzymatic studies on planar supported membranes using a widefield fluorescence LAURDAN Generalized Polarization imaging approach", Biochim. Biophys. Acta - Biomembranes, 1859: 888-895 view►
  • A. Bagatolli and R. P. Stock. 2016. "The cell as a gel: material for a conceptual discussion" Physiological Mini Reviews 9(5): 38-49 view►
  • Malacrida, S. Astrada, A. Briva, M. Bolatti-Fogolín, E. Gratton and L. A. Bagatolli. 2016. "Spectral Phasor analysis of LAURDAN fluorescence in live A549 lung cells to study the hydration and time evolution of intracellular lamellar bodies-like structures." Biochim. Biophys. Acta - Biomembranes, 1858(11):2625-2635 view►
  • Bhatia, F. Cornelius, J. Brewer, L. A. Bagatolli, A. C. Simonsen, J. H. Ipsen, and O. G. Mouritsen. 2016. "Spatial distribution and activity of Na+/K+-ATPase in lipid bilayer membranes with phase boundaries" Biochim. Biophys. Acta - Biomembranes 1858:1390-1399 view►
  • Leurgans, M. Bloksgaard, J. Brewer, L.A. Bagatolli, M. Fredgart, K. Rosenstand, M. Lyck Hansen, L. Melholt Rasmussen, A. Irmukhamedov, and J. De Mey. 2016."Endothelin-1 Shifts the Mediator of Bradykinin-Induced Relaxation from NO to H2O2 in Resistance Arteries from Patients with Cardiovascular Disease" British Journal of Pharmacology 173(10):1653-1664 view►

Book chapters

  • A. Bagatolli. "Fluorescence spectroscopy: basic foundations and methods." In Analytical Techniques in Delivery Science and Technology A. Müllertz, Y. Perrie, and T. Rades (Eds) Elsevier, chapter 2, pp. 29-60 (2016). view►
  • A. Bagatolli and R. P. Stock. "The use of 6-acyl-2-(dimethylamino)naphtalenes as relaxation probes of biological environments" In Perspectives on Fluorescence: A Tribute to Gregorio Weber, D.M Jameson Ed. Springer Series on Fluorescence, pp. 197-216 (2016) view►
  • B. Nielsen and L.A. Bagatolli "Storage conditions of skin affect tissue structure and in vitro percutaneus penetration" Humbert P, Maibach H, Fanian F et al. In Agache's Measuring the Skin. Second edition. Springer International Publishing, pp 1191-1195 (2017). view►
  • A.Bagatolli. "Optical microscopy techniques and giant unilamellar vesicles" Chapter 10, In "The giant vesicle book. C. Marques and R. Dimova eds. CRC press, Francis & Taylor group (2019). view►

Books

  • Vida ¿una cuestión de grasas? Una perspectiva desde la biofísica de membranas; edición electronica revisada (2017). Luis A. Bagatolli y Ole G. Mouritsen. Yachay EP, Ecuador. ISBN 978-987-42-6564-7 descargar► (in Spanish)
  • Life -as a matter of fat: Lipids in a membrane biophysics perspective (2016). Ole G. Mouritsen and Luis A. Bagatolli. Springer. ISBN 978-3-319-22614-9 view►

Popular dissemination

  • A. Bagatolli. 2018. “La célula como un gel: ¿Un cambio de paradigma necesario para reinterpretar como ocurren los procesos celulares?” view► (in spanish)