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RESEARCH

In parasites, efficiency to react to changes in the microenvironment is directly related to their survival odds. Posttranslational protein modifications enable cells to rapidly and specifically respond to endogenous or exogenous stimuli, avoiding time- and energy-consuming de novo protein synthesis. Our laboratory studies different post translational modifications of proteins (methylation, cytrullination, SUMOylation, palmitoylation) during Giardia lamblia encystation to determine the similarities as well as differences with eukaryotic cells. Those differences will be employed to exploit putative new drugs to treat giardiasis.

 

RESEARCH SUPPORT

2016-2020. PICT 2015-0154. FONCYT.ROLE: PI $ 740.244,00
2018-2021. SECYT. ROLE: PI $ 88.000,00
2019-2022. PICT 2017-0464. FONCYT. ROLE: PI $ 960.000,00

PhD STUDENTS

Luciano Diaz Perez 

Veterinarian

Doctoral Fellow. FONCYT

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SELECTED PUBLICATIONS

  1.  2002. Simultaneous expression of different variant-specific surface proteins in single Giardia lamblia trophozoites during encystation. Carranza, P.G., Feltes, G., Rópolo, A, Quintana, S. M. C, Touz, M. C., and Luján, H.D. Infection and Immunity, 70, 5265-5268.
  2.  2005. Identification of Variant-specific surface proteins in Giardia muris trophozoites. Rópolo A, Saura A, Carranza P and Lujan HD. Infection and Immunity. Vol 73, 5208-5211.
  3.  2006. Presence of neutrophils bearing antigen in lymphoid organs of immune mice. Maletto B*, Rópolo A*, Alignani D, Liscovsky M, Ranochia R, Morón G and Pistoresi MC. Blood. 2006. 108: 3094-3102. *Equal contributors.
  4. 2008. Arginine deiminase plays multiple regulatory roles in the biology of Giardia lamblia. Touz MC*, Rópolo A*, Rivero R, Vranych C, Conrad J. Svard S and Nash TE. Journal of Cell Science. 121: 2930-2938. *Equal contributors.
  5. 2009. KIF4 mediates anterograde translocation and positioning of ribosomal constituents to axons. Bisbal M, Wojnacki J, Jausoro I, Peretti D, Rópolo A, Sesma J and Cáceres AJ. Biochem Chem. 284(14): 9489-97.
  6.  2010. Adaptor Protein 2 Regulates Receptor-Mediated Endocytosis and Cyst Formation in Giardia lamblia. Maria Romina Rivero, Cecilia Veronica Vranych, Mariano Bisbal, Belkys Maletto, Andrea Rópolo, and Maria Carolina Touz. Biochemical Journal. 428 (1): 33-45.
  7.  2010. A lesson in survival, by Giardia lamblia. Andrea Rópolo and María Carolina Touz. TheScientificWorldJournal 10: 2019-2031.
  8.  2011. Giardia lamblia low-density lipoprotein related-receptor is involved in selective lipoprotein endocytosis and parasite replication. María Romina Rivero, Silvana Miras, Rodrigo Quiroga, Andrea Silvana Rópolo and María Carolina Touz. Mol Microbiol. 79 (5): 1204-1219
  9.  2011. Immunodominant proteins alpha-1 giardin and beta-giardin are expressed in both assemblages A and B of Giardia lamblia. Constanza Feliziani, María Cecilia Merino, María Romina Rivero, Ulf Hellman, María Cristina Pistoresi and Andrea S. Rópolo. BMC Microbiology. 2011. 11:233.
  10. 2012. SUMOylation in Giardia lamblia: a conserved post-translational modification in one of the earliest divergent eukaryotes. Cecilia Vranych, María Cecilia Merino, Nahuel Zamponi, María Carolina Touz and Andrea Rópolo. Biomolecules. Chapter: Protein SUMOylation. 2 (3), 312-331.
  11.  2012. Vacuolar protein sorting receptor in Giardia lamblia. María Romina Rivero, Silvana Miras, Constanza Feliziani, Nahuel Zamponi, Stanley Hayes, Andrea S Rópolo and María Carolina Touz. PloS One, 7 (8): e43712.
  12. 2013. The giardial VPS35 retromer subunit is necessary for multimeric complex assembly and interaction with the Vacuolar protein sorting receptor. Silvana Lorena Miras, María Cecilia Merino, Natalia Gottig, Andrea Rópolo and María Carolina Touz. BBA Molecular Cell Research, 1833 (12): 2628-2638.
  13.  2013. SUMOylation and deimination of proteins: two epigenetic modifications involved in Giardia encystation. Cecilia Vranych, Romina Rivero, María Cecilia Merino, Gonzalo Mayol, Nahuel Zamponi, Belkys Maletto, María Cristina Pistoresi, María Carolina Touz and Andrea Rópolo. BBA Molecular Cell Research 1843, 1805-1817.
  14. 2014. Identification of Giardia lamblia DHHC Proteins and the role of protein S- palmitoylation in the  ncystation process. María C. Merino, Nahuel Zamponi, Cecilia Vranych, María C. Touz and Andrea Rópolo. Plos Negl Trop Dis 8 (7): e2997.
  15.  2015. The giardial ENTH protein participates in lysosomal protein trafficking and endocytosis. Constanza Feliziani, Nahuel Zamponi, Natalia Gottig, Andrea Rópolo, Adriana Lanfredi-Rangel, María Carolina Touz. BBA Molecular Cell Research 1854, 646-659.
  16.  2017. Agostina Salusso, Natacha Zlocowski, Gonzalo Mayol, Nahuel Zamponi and Andrea S. Rópolo. Histone methyltransferase 1 regulates the encystation process in the parasite Giardia lamblia. FEBS Journal. 284 (15) 2396-2409.
  17. 2018. María Carolina Touz, Constanza Feliziani, Andrea Rópolo. Membrane-Associated Proteins in Giardia lamblia. Genes (Basel). Aug 10;9(8). pii: E404. doi: 10.3390/genes9080404. Review.
  18.  2018. Lorena Frontera, Sofía Moyano, Gonzalo Quassollo, Adriana Lanfredi-Rangel, Andrea Rópolo and María Carolina Touz. Lactorerrin and lactoferricina endocytosis halt Giardia cell growth and prevent infective cyst production. Scientific Reports. 21;8 (1) 18020.
  19. 2019. Andrea Rópolo, Constanza Feliziani and María Carolina Touz. Unusual proteins in Giardia duodenalis and their role in survival. Adv Parasitol, 106: 1-50.
  20. 2019. Sofía Moyano, Juliana Musso, Constanza Feliziani, Nahuel Zamponi, Lorena Frontera, Andrea Rópolo, Adriana Lanfredi-Rangel, Marco Lalle and María C. Touz. Exosome biogenesis in the protozoa parasite Giardia lamblia: a model of reduced interorganellar cross-talk. Cells 2019, 8, 1600; doi:10.3390/cells8121600
  21. 2020. Gonzalo Mayol, Victoria Revuelta, Agostina Salusso, María Carolina Touz and Andrea Rópolo. Evidence of nuclear transport mechanisms in the protozoan parasite Giardia lamblia. BBA Mol Cell Res. 1867 (1): 118566.