Membrane traffic and cytoskeleton regulation in neuronal development

The development and maintenance of neuronal polarity involves the coordination of
several cellular events such as the assembly of actin and microtubules cytoskeleton; the
sorting and delivery of membrane proteins from Golgi apparatus, and the trafficking of
these membrane and cytoskeletal components and the addition of membrane at sites of
active growth in specialized regions of the neuron. These mechanisms are mainly
regulated by the activity of several members of the Rho GTPases family. Together with
their effector proteins, these small GTPases control different aspects of neuronal
morphology. The main interest of our laboratory is to study the molecular and cellular
mechanisms that regulate and integrate these events. Particularly, we are exploring the
role of the atypical GTPase RhoD, an important regulator of cytoskeleton dynamics and
membrane trafficking, through effectors and signaling pathways different from those
observed for classical Rho GTPases Rac1, Cdc42 and RhoA. Likewise, we are interested in
studying the mechanisms that regulate the positioning and polarized distribution of the
Golgi apparatus and the biosynthetic pathway in neurons and their effect on the
development and maintenance of the dendritic compartment.


Josefina Inés Martín

Estudiante de Doctorado Ciencias Biológicas, UNC.

Becario doctoral FONCyT

 Gala Valletto
Undergraduate Student (FCEFyN-UNC)


Research grant. PID 2018. MinCyT-Córdoba, Argentina. Research grant. ANPCyT, PICT-D. MinCyT-
Argentina. Research grant. ANPCyT, PICT-Class V. MinCyT-Argentina. Bilateral Cooperation
Programe CONICET (Argentine)-CNR (Italy).

PUBLICATION (Last 5 years)

  1. Bisbal M*, Sanchez M* (2019) Neurotoxicity of the Pesticide Rotenone on neuronal
    polarization: a mechanistic approach. Neural regeneration research 14:762-6. (*co-
    corresponding authors).
  2. Peris L* ,# , Bisbal M* ,# , Hernandez-Martinez J, Saoudi Y, Jonckheere J, Rolland M, Sebastien
    M, Brocard J, Denarier E, Bosc C, Guerin C, Gory-Fauré S, Deloulme C, Lante F, Arnal I,
    Buisson, Goldberg Y, Blanchoin L, Delphin C, Andrieux A # (2018) A critical function for
    Microtubule-Associated Protein 6 in activity-dependent stabilisation of actin filaments in
    dendritic spines. Nature communication 9(1):3775 (*equally contributing authors; # co-
    corresponding authors).
  3. Bisbal M*, Remedi M, Quassollo G, Caceres A, Sanchez M* (2018). Rotenone inhibits
    axonogenesis via an Lfc/RhoA/ROCK pathway in Cultured hippocampal neurons. Journal of
    neurochemistry 146(5):570-584. (*co-corresponding authors).
    Unsain N, Bordenave M, Martinez G, Jalil S, von Bilderling C, Barabas F, Masullo L,
    Johnstone A, Barker P, Bisbal M, Stefani F, Caceres A (2018) Remodeling of the
    Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin
    Decrease during Axonal Degeneration. Scientific reports 8(1):3007.
    Zamponi E, Buratti F, Cataldi G, Caicedo HH, Song Y, Jungbauer LM, LaDu MJ, Bisbal M,
    Lorenzo A, Ma J, Helguera PR, Morfini GA, Brady ST, Pigino GF (2017) Prion protein inhibits
    fast axonal transport through a mechanism involving casein kinase 2. PloS one
  4. Quiroga S, Bisbal M, Cáceres A (2017) Regulation of plasma membrane expansion during
    axon formation. Developmental neurobiology 78(3):170-180.
  5. Bisbal M, Quassollo G, Caceres, A (2016) “Imaging Golgi outposts in fixed and living
    neurons” Methods Mol Biol. 1496: 31-39.
  6. Quassollo G, Wojnacki J, Salas, DA, Gastaldi L, Marzolo MP, Conde C, Bisbal M, Couve A.,
    Caceres A (2015) A RhoA Signaling Pathway Regulates Dendritic Golgi Outpost Formation.
    Current biology. 25(8):971-82.
  7. Daoust A, Saoudi Y, Brocard J, Collomb N, Batandier C, Bisbal M, Salome M, Andrieux A,
    Bohic S, and Barbier EL (2014). Impact of manganese on primary hippocampal neurons
    from rodents. Hippocampus. 24(5):598-610