Membrane traffic and cytoskeleton regulation in neuronal development

The development and maintenance of neuronal polarity involves the coordination of several cellular events such as the assembly of actin and microtubules cytoskeleton; the sorting and delivery of membrane proteins from Golgi apparatus, and the trafficking of these membrane and cytoskeletal components and the addition of membrane at sites of active growth in specialized regions of the neuron. These mechanisms are mainly regulated by the activity of several members of the Rho GTPases family. Together with their effector proteins, these small GTPases control different aspects of neuronal morphology. The main interest of our laboratory is to study the molecular and cellular mechanisms that regulate and integrate these events. Particularly, we are exploring the role of the atypical GTPase RhoD, an important regulator of cytoskeleton dynamics and membrane trafficking, through effectors and signaling pathways different from those observed for classical Rho GTPases Rac1, Cdc42 and RhoA. Likewise, we are interested in studying the mechanisms that regulate the positioning and polarized distribution of the Golgi apparatus and the biosynthetic pathway in neurons and their effect on the development and maintenance of the dendritic compartment.



Gala Valletto

BS in Biological Sciences student. National University of Córdoba (UNC).


FONCyT PICT-D 2017, MinCyT-Córdoba PID 2018, FONCyT PICT Clase V 2014, Programa de
Cooperación Bilateral (PCB I) CONICET-CNR (Consiglio Nazionale delle Ricerche Italia).
Return Home Award: Category 1C ISN (International Society for Neurochemistry).

PUBLICATION (Last 5 years)

  1. Bisbal M*, Sanchez M* (2019) Neurotoxicity of the Pesticide Rotenone on neuronal
    polarization: a mechanistic approach. Neural regeneration research 14:762-6. (*co-
    corresponding authors).
  2. Peris L* ,# , Bisbal M* ,# , Hernandez-Martinez J, Saoudi Y, Jonckheere J, Rolland M, Sebastien
    M, Brocard J, Denarier E, Bosc C, Guerin C, Gory-Fauré S, Deloulme C, Lante F, Arnal I,
    Buisson, Goldberg Y, Blanchoin L, Delphin C, Andrieux A # (2018) A critical function for
    Microtubule-Associated Protein 6 in activity-dependent stabilisation of actin filaments in
    dendritic spines. Nature communication 9(1):3775 (*equally contributing authors; # co-
    corresponding authors).
  3. Bisbal M*, Remedi M, Quassollo G, Caceres A, Sanchez M* (2018). Rotenone inhibits
    axonogenesis via an Lfc/RhoA/ROCK pathway in Cultured hippocampal neurons. Journal of
    neurochemistry 146(5):570-584. (*co-corresponding authors).
    Unsain N, Bordenave M, Martinez G, Jalil S, von Bilderling C, Barabas F, Masullo L,
    Johnstone A, Barker P, Bisbal M, Stefani F, Caceres A (2018) Remodeling of the
    Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin
    Decrease during Axonal Degeneration. Scientific reports 8(1):3007.
    Zamponi E, Buratti F, Cataldi G, Caicedo HH, Song Y, Jungbauer LM, LaDu MJ, Bisbal M,
    Lorenzo A, Ma J, Helguera PR, Morfini GA, Brady ST, Pigino GF (2017) Prion protein inhibits
    fast axonal transport through a mechanism involving casein kinase 2. PloS one
  4. Quiroga S, Bisbal M, Cáceres A (2017) Regulation of plasma membrane expansion during
    axon formation. Developmental neurobiology 78(3):170-180.
  5. Bisbal M, Quassollo G, Caceres, A (2016) “Imaging Golgi outposts in fixed and living
    neurons” Methods Mol Biol. 1496: 31-39.
  6. Quassollo G, Wojnacki J, Salas, DA, Gastaldi L, Marzolo MP, Conde C, Bisbal M, Couve A.,
    Caceres A (2015) A RhoA Signaling Pathway Regulates Dendritic Golgi Outpost Formation.
    Current biology. 25(8):971-82.
  7. Daoust A, Saoudi Y, Brocard J, Collomb N, Batandier C, Bisbal M, Salome M, Andrieux A,
    Bohic S, and Barbier EL (2014). Impact of manganese on primary hippocampal neurons
    from rodents. Hippocampus. 24(5):598-610